目的:建立HPLC法測定果汁中9種有機酸的檢測方法。

果汁中9種有機酸的反相高效液相色譜法測定 
   方法:果汁樣品經(jīng)稀釋過濾后,采用Gemini C18色譜柱(4.6 mm×250 mm,5μm)分離,以0.01 mol/L磷酸氫二銨溶液(用磷酸調(diào)節(jié)pH=2.6)作為流動相,UV210 nm檢測,外標(biāo)法定量。結(jié)果:9種有機酸的線性范圍分別為草酸1.0~400 mg/L、酒石酸2.0~500 mg/L、丙酮酸1.0~500 mg/L、蘋果酸5.0~2000 mg/L、抗壞血酸1.0~400 mg/L、乙酸8.0~2000 mg/L、檸檬酸5.0~2000 mg/L、富馬酸0.5~100 mg/L、反烏頭酸1.0~400 mg/L,各種有機酸的線性相關(guān)系數(shù)r>0.999,回收率在93.7%~109.0%,RSD均小于5.0%,最低檢測限在0.2~7.5 mg/L范圍。結(jié)論:該方法具有快速、靈敏、簡便、準(zhǔn)確等優(yōu)點,可應(yīng)用于果汁中有機酸的檢測,能滿足實際工作的需要。
    Objective: To develop an analytical method for the simultaneous determination of nine organic acids in juice beverages by high-performance liquid chromatography(HPLC).Methods:The juice beverage samples were diluted by water and filtered in the sample vials.Then the nine objects were separated on Gemini C18 column(250 mm×4.6 mm i.d.,5 μm)by using a mobile phase of 0.01 mol/L(NH4)2HPO4(adjusted pH to 2.6 by H3PO4) at a flow rate of 1.0 ml/min.Detection was performed on an ultraviolet detector at λ=210 nm and ...